I have analysed ethyl acetate and methanol extracts by using GC-MS. It's worth interesting to note that these extracts revealed high contents of total phenols, tanins and carotenoids. However, while caracterising these organic extracts, i haven't detected phenolic …
The ability of P. hexandrum ethyl acetate extract to scavenge hydrogen peroxide was evaluated according to the method of [23, 24]. A solution of H2O2 (2 mmole) was prepared in phosphate buffer (pH 7.5). Plant extract (50–300 μg/mL) were added to the hydrogen peroxide solution (0.6 mL).
From the different crude extracts, the crude "ethyl acetate" extract was then partitioned by double distilled water and ethyl acetate to obtain water fraction (WF) and ethyl acetate fraction (E.AF). For checking the antioxidant activity, each extract/fraction was dried and redissolved in methanol.
The TLC chromatograms of the ethyl acetate extract are shown in Figures Figures1a1a and andb.b. Comparing the compound profiles, TLC chromatogram developed in EMW showed 3 compounds under UV light (wavelength 302 nm), 1 compound under UV light (wavelength 365 nm), and 4 compounds after spraying with vanillin reagent.
It was observed that extracts from Datura metel plant which were formed using methanol, ethyl acetate, acetone and hexane shows the maximum antioxidant activity with 41.2±0.64, 40.8±0.69, 38±1.35 and 40.9±0.14% inhibition respectively followed by distilled water (31.9±0.23% inhibition) which is significantly higher than chloroform extract (only 15.3±0.14% inhibition) (Fig. 1, Table 3).
Aug 29, 2010· I tested compounds for solubility in water, NaOH and HCl. Ethanol was miscible in all so was ethylamine. what confuses me is that ethylacetate has the same Dipole moment and relative solubility in water as phenol but phenol is miscible in water why ethyl acetate is not. Another point is that ethyl acetate is a polar molecule. pls enlighten
Jul 18, 2012· The fractionation of the extract afforded two known compounds: lupeol (1) and aurantiamide acetate (2) together with two mixtures of fatty acids: oleic acid and n-hexadecanoic acid (B1); n-hexadecanoic acid, octadecanoic acid and docosanoic acid (B2). Aurantiamide acetate was the most active compound.
Feb 04, 2014· Hexane, ethyl acetate, methanol and water extracts of C. cujete leaves were investigated against bacterial strains. DPPH radical scavenging, antioxidant activity by β-carotene bleaching test and cytotoxic activity of the methanol extract of aerial parts of this plant were evaluated by David et al.. However, according to our knowledge, detailed antioxidant activities on different fractions of leaves …
Inhibition percentage of both extracts at 65% was known to occur at concentration of 1000 ppm for ethyl acetate extract and 2000 ppm for ethanolic extract. An important finding was that ethyl acetate can be used as appropriate solvent for extracting antioxidant compound better than ethanolic. In conclusion, the mycelium extract of Ganoderma sp. extracted with ethyl acetate and ethanol as solvent is potential to …
In phytochemical screening of ethyl acetate extract showed positive test for Flavonoids, Tannins, Saponins, Phenolic compounds. Phenol, flavonoid metabolites are correlated with the antioxidant activity and these metabolite have direct role in freeradical scavenging activity 25 .